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KMID : 0545119950050020074
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 2 p.74 ~ p.79
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Genetic analysis of polyketide biosynthetic genes isolated from Streptomyces albus, a salinomycin producer.
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KWON, HYOUNG JIN
HUTCHINSON, C.R./JIN, HYUNG-JONG/KIM, SOO-UN/LEE, KYE-JOON/SUH, JOO-WON
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Abstract
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Sequence analysis of a DNA region encompassing the site of hybridization to actl, the gene for type Ⅱ minimal polyketide synthase (PKS) for actinorhodin biosynthesis, from Streptomyces ablus revealed three more complete open reading frames additional to the already found two genes, plausibly encoding β-ketoacyl synthase/acyl transferase (KS/AT) and chain length determining factor (CLF). The open reading frames (ORFs) were named salA, salD, and salE, from the upstream. In the homology analysis of the deduced amino acid sequences, SalA resembles the Streptomyces glaucescens Tcml, decaketide cyclase, SalD resembles acyl carrier protein in typeⅡ PKS, and SalE resembles the ActⅢ ketoreductase. The whole 4.4kb of DNA sequence obeys the same conservation pattern as other type Ⅱ PKSs. Therefore, we suggest that the 4.4 kb DNA from Streptomyces albus encompasses genes encoding enzymes for polyketide biogenesis in the organism and its organization is type Ⅱ. The exsitence of SalA, an analogue of the aromatic cyclase, revealed a relatedness of the 4.4 kb DNA with the aromatic PKS.
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